Solid-phase extraction of N-linked glycopeptides
نویسندگان
چکیده
منابع مشابه
Synthesis of N-linked glycopeptides via solid-phase aspartylation.
An efficient strategy for the preparation of N-linked glycopeptides is described. The method relies on the use of side chain protecting groups on aspartic acid residues, namely the allyl and Dmab esters, which are orthogonal to those utilised in Fmoc-strategy SPPS. After peptide assembly these protecting groups were selectively removed and the resulting free side chains derivatised with a glyco...
متن کاملIodination on tyrosine residues during oxidation with sodium periodate in solid phase extraction of N-linked glycopeptides.
Solid-phase extraction of N-linked glycopeptides (SPEG) using hydrazide-modified supports has become a common sample preparation procedure in glycoproteomic experiments. We demonstrate that iodination of tyrosine residues occur in SPEG as a side reaction during an oxidation step with sodium periodate. MS/MS analysis of oxidized bovine serum albumin and carbonic anhydrase digests revealed a char...
متن کاملEfficient use of the Dmab protecting group: applications for the solid-phase synthesis of N-linked glycopeptides.
An efficient protocol for the chemoselective removal of Dmab esters on the solid phase is reported; this method has been successfully utilised for the convergent solid phase synthesis of N-linked glycopeptides.
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objective: Phenytoin is an anti-seizure medication used to treat epilepsy, as well as to control arrhythmias (irregular heartbeat) and to treat migraine headaches and nerve pain. It is recommended to determine the amount of this drug in the blood to control the seizure and prevent its toxicity. In the present study, a sensitive and simple procedure based on the dispersive solid phase extraction...
متن کاملIntroduction to Solid-phase Extraction
We have a sample in front of us. It is of unknown composition, but we know that it is complex, containing anywhere from a few hundred to many thousand chemical components. It is of uncertain physical form perhaps it looks like a liquid, but it has solid particles floating in it. We think that a particular compound is present in that sample, somewhere and in some form, but we don't know how much...
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ژورنال
عنوان ژورنال: Nature Protocols
سال: 2007
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2007.42